Intangiriro

Muri laboratwari zigezweho z’ubuvuzi n’imiti,Imiyoboro yo gucukura ikoreshwa cyane mu gucukura uturemangingo, gukora ibikorwa bya shimi, kubika ingero n'ibindi bikorwa by'ingenzi.Akamaro kabyo mu igerageza ntigashobora kwirengagizwa. Kubera ko utu duce tw’igerageza duhura neza n’ibipimo by’igerageza, ibikoresho byabyo, ingano yabyo, gufunga kwabyo ndetse n’iyo byaba byarasizwe umuti cyangwa bitarasizwe bizagira ingaruka zikomeye ku bisubizo by’igerageza. Guhitamo nabi bishobora gutuma habaho kwanduzwa, gutakaza ingero cyangwa kubogama kw’amakuru y’igerageza, bityo bigatera ingaruka ku kuri no kongera kugaragara kw’ubushakashatsi.

Ubwoko bw'ingenzi bw'imiyoboro y'amazi ikoreshwa mu gucukura

Hari ubwoko bwinshi bw'imiyoboro yo gucukura ikoreshwa rimwe, kandi abashakashatsi bagomba guhitamo ubwoko bukwiye hakurikijwe intego y'igerageza, aho imikorere ibera ndetse n'imiterere y'ingero. Zishyizwe mu byiciro bitatu bikurikira: ibikoresho, ubushobozi n'imikorere yihariye:

1. Gushyira mu byiciro hakurikijwe ibikoresho

Imiyoboro yo gucukura ikoreshwa mu bikoresho bitandukanye itandukanye mu kurwanya ubushyuhe, kudahinduka kw'imiti n'imiterere y'urumuri:

• Polypropylenee: Ubudahangarwa bwiza ku bushyuhe bwinshi n'ubushyuhe bw'ibinyabutabire, bukwiriye gukoreshwa mu guteza imbere uturemangingo dusanzwe, mu igerageza rya biology ya molekile n'izindi porogaramu.
• Polystyrene: ubwisanzure buri hejuru, byoroshye kubona imiterere y'amazi n'uturemangingo, bikunze gukoreshwa mu isuzuma ry'amaso, ariko ntibishobora kurwanya ubushyuhe bwinshi, ubusanzwe ntibishobora gukoreshwa mu kwimura ibintu mu buryo bworoshye.
• Imiyoboro yo gukurura ikirahureNubwo zishobora kongera gukoreshwa kandi zigahinduka mu buryo burambye, zirahenze, zisaba isuku n'uburyo bwo gusukura no gukaraba, kandi zishobora gutera ibyago byo kwanduzwa n'izindi.

2. Gushyira mu byiciro hakurikijwe ubushobozi

Bitewe n'ingano y'icyitegererezo gikenewe mu igerageza, ubushobozi bw'imiyoboro yo gukurura ingano buri hagati y'ingano nto n'inini:

• Imiyoboro ya microcentrifuge: ikoreshwa cyane mu gutanga ingero, gutanga amazi aturuka kuri centrifugal, gukuramo ADN/RNA n'ibindi bikorwa.
• Imiyoboro isanzwe yo gukwirakwiza ibiribwa: ubushobozi bukoreshwa cyane muri laboratwari, bukwiriye ubuhinzi bw'uturemangingo, kuvanga uburyo bwo gukora, kubika ingero n'ibindi bikorwa.
• Imiyoboro minini yo gucukuramo ibintu: ikwiriye ubuhinzi bw'uturemangingo tunini cyangwa gutunganya byinshi mu buryo bw'imiti.

3. Gushyira mu byiciro hakurikijwe imikorere yihariye

Utwo duce turaboneka dufite ibindi bintu byinshi bidufasha guhaza ibyo dukeneye mu igerageza:

• Imiyoboro ya Aseptic Pre-sterilized: uruganda rwasuzumwe hakoreshejwe imirasire ya gamma cyangwa autoclaving, ikwiriye gukoreshwa mu igerageza rifite ibisabwa byinshi byo gusesereza.
• Ifite umupfundikizo wa karitoli: yemerera ihindagurika rya gaze, rikwiriye udukoko cyangwa utunyangingo dukenera umwuka kandi rikarinda kwanduzwa n'ibintu biva hanze.
• Imiyoboro Irwanya Ubushyuhe Buke: ishobora gukoreshwa neza mu kirere kirimo azote y'amazi -80°C cyangwa ndetse no mu bidukikije, ikwiriye mu kubika ingero z'ibinyabuzima mu bushyuhe buke igihe kirekire.
• Ibyuma byarangije kwiga cyangwa bitarangije kwiga: Imiyoboro yarangije gukoreshwa ni yo yoroshye gupima no gutanga ingano y'amazi vuba kugira ngo yongere imikorere myiza y'igerageza.

Ibintu by'ingenzi mu guhitamo imiyoboro y'umuco

Mu gihe cyo gushushanya no gukora igerageza, guhitamo imiyoboro ikwiye yo gucukura ikoreshwa rimwe na rimwe ni ingenzi cyane kugira ngo habeho imikorere myiza n'ukuri kw'ibisubizo by'igerageza. Abashakashatsi bagomba gusuzuma mu buryo bwuzuye ibintu byinshi by'ingenzi:

1. Ubwoko bw'igerageza

Ibisabwa ku miyoboro yo gucukura biratandukanye cyane bitewe n'igerageza kandi bigomba gutoranywa hakurikijwe ibikubiye mu gikorwa n'aho igerageza ryabereye:

• Ku bijyanye n'ubuhinzi bw'uturemangingo: ibisabwa kugira ngo umuntu atandura ni byinshi cyane, kandi gukoresha imiyoboro yangiritse mbere yo gusukura ifite imipfundikizo y'imashini ihumeka kugira ngo habeho ihindagurika rya gaze.
• Igerageza rya PCR / biology ya molekile: hakenewe ko nta enzymes za ADN, enzymes za RNA n'imiyoboro idafite pyrogen, akenshi hakoreshwa imiyoboro ya polypropylene isukuye.
• Ububiko bw'ubushyuhe buri hasi: Imiyoboro ifite ubushobozi bwo kwirinda ubushyuhe buke igomba gukoreshwa kugira ngo hirindwe ko ihinduka mu bushyuhe buke.

2. Ingero z'ibiranga

Imiterere ya fizikike y'icyitegererezo igira ingaruka ku buryo butaziguye ku guhitamo ibikoresho n'imikorere y'imiyoboro yo gukwirakwiza ibiribwa:

• Ingero z'amazi cyangwa zikomeye: igena ingano ikenewe n'imiterere y'agace k'umuyoboro w'amazi.
• Ingero za aside cyangwa alkaline: ingero zangiza cyane zikenera ibikoresho birwanya imiti kugira ngo hirindwe kwangirika cyangwa kwanduzwa n'imiyoboro y'amazi.
• Niba ari ngombwa kwirinda urumuri: Ingero z’urumuri zigomba guhitamo umuyoboro w’ubutare bw’umuhondo cyangwa butagaragara, kugira ngo hirindwe kwangirika kw’ifoto.

3. Ibisabwa mu isuku

Gukenera koroshya isuku n'ubwoko bwo koroshya isuku bikoreshwa ni ibintu by'ingenzi mu guhitamo imiyoboro:

• Kwihagarika mbere yo gufunga umubiri ugereranije no kwihagarika ubwawe: Ibikoresho byakozwe mbere yo gusukura ibyuma bikoreshwa mu ruganda bikwiranye n'igerageza rigezweho, bigabanya igihe; niba laboratwari ifite ibikoresho byo gusukura ibyuma bya autoclave, ushobora guhitamo imiyoboro ya PP ishobora gusukura ibyuma bya autoclave.
• Guhuza uburyo bwo gufunga umubiriUrugero, ibikoresho bya PS ntibikwiriye gukoreshwa mu buryo bworoshye kandi bikoreshwa rimwe gusa.

4. Guhuza ibintu

Imiyoboro igomba kuba ijyanye neza n'ibikoresho bya laboratwari kugira ngo ikore neza kandi itange ibisubizo byizewe:

• Guhuza hagati y'ibice bibiri: Imiyoboro ikoreshwa mu gukurura umwuka igomba kuba ikomeye mu miterere yayo kugira ngo ihangane n'imbaraga za centrifugal ku muvuduko mwinshi wo kuzenguruka.
• Guhuza imikorere y'ikoranabuhanga: Ku igerageza risaba gukoresha robo zikoresha imiyoboro, sisitemu zikoresha ikoranabuhanga, nibindi, ingano z’imiyoboro zigomba gukoreshwa.

5. Ikiguzi n'uburyo burambye

Gugenzura neza ibiciro n'imikoreshereze y'umutungo nabyo ni ingenzi mu guhaza ibyifuzo by'igerageza:

• Ishobora gukoreshwa cyangwa ikoreshwa mu buryo butari ubwa mbere: imiyoboro ikoreshwa rimwe na rimwe yoroshye kuyicunga no kwirinda kwanduzanya, ikwiriye gukoreshwa mu igerageza rikoresha imbaraga nyinshi; imiyoboro y'ibirahure ishobora kongera gukoreshwa ikwiriye gukoreshwa mu igerageza ry'ibanze ridafite ingengo y'imari ihagije.
• Igipimo cy'ibicuruzwa: kugura ibintu byinshi bishobora kugabanya igiciro cy'ibikoresho, bikaba bikwiye imishinga minini kandi y'igihe kirekire; imiterere mito kandi yihariye irahindagurika ariko ihenze cyane.

Inama ku birebana n'ikoreshwa rusange ry'ibipimo

Dukurikije ibyo ubwoko butandukanye bw'igerageza bukeneye, utuyoboro dukurikira two gucukura dukoreshwa mu buryo butandukanye turasabwa mu bihe bitandukanye bikunze gukoreshwa, tugamije kunoza imikorere n'ubwizerwe bw'igerageza:

1. Guhinga utunyangingo

• Ubwoko busabwa: imiyoboro ya polypropylene idahumanye ifite imipfundikizo y'akabati gahumeka
• Impamvu: Ibikoresho bya polypropylene bifite ubushobozi bwo kudakora neza mu binyabutabire no guhuza neza n'ibinyabuzima, bikwiranye n'amazi yo mu bwoko bwa selile. Igipfukisho cya cartridge gishobora guhanahana neza gaze, kikarinda kwanduza mikorobe, kandi kigahura n'ibidukikije bikenewe kugira ngo selile zigwire.

2. Gusobanukirwa molekile za PCR/qPCR

• Ubwoko bw'ibisabwa: imiyoboro yihariye ya PCR idafite nuclease, idafite pyrogen cyangwa imiyoboro ya microcentrifuge
• Impamvu: Imiyoboro ya polypropylene isukuye cyane, nyuma yo kuvurwa neza, ishobora kwirinda kwangirika cyangwa kwanduzwa n'ingero, kugira ngo habeho ibisubizo nyabyo kandi byizewe byo kongera imbaraga. Igishushanyo mbonera cy'urukuta gito kirasabwa kugira ngo hongerwe imikorere myiza yo kohereza ubushyuhe.

3. Ububiko bw'ubushyuhe buri hasi

• Ubwoko bw'ingenzi: Umuyoboro wa Polypropylene udashyuha cyane, ufite imiterere irwanya gukonjesha n'umupfundikizo wo gufunga screw
• Impamvu: Iyi miyoboro ishobora gukoreshwa mu kubika ingero igihe kirekire kuri -80°C cyangwa ndetse no muri azote y'amazi, kandi imiterere yihariye irinda umuyoboro gucika intege no kuva kw'ingero. Ikwiriye kubikwa igihe kirekire k'uturemangingo, ingero z'amaraso, poroteyine cyangwa aside nucleic.

4. Centrifuge

• Ubwoko bw'ibisabwa: imiyoboro ya polypropylene yihanganira cyane, ifite imiterere y'inyuma izengurutse cyangwa ifite ishusho y'urukiramende, ifite rotors za centrifuge
• Impamvu: Imiyoboro ya PP ifite ubushobozi bwo guhangana n’imiyoboro ya centrifugal kandi ishobora kwihanganira imbaraga nyinshi za centrifugal idahinduka cyangwa ngo ivunike. Igice cyo hasi cy’urukiramende gifasha gukusanya uturemangingo cyangwa kumanuka hagati no kongera umuvuduko wo kugarura ibintu.

Amakosa asanzwe n'uburyo bwo kuyarinda

Mu by’ukuri, gutsindwa kw’igerageza bitewe no guhitamo nabi imiyoboro yo gukwirakwiza amazi bibaho rimwe na rimwe. Amakosa menshi akunze kugaragara hamwe n’ibitekerezo bijyanye n’ibisubizo byatanzwe n’abashakashatsi ni ibi bikurikira:

1. Gukoresha nabi ibikoresho bidashyuha cyane mu gufunga by'agateganyo