Intangiriro

Muri laboratwari zigezweho za biomedical na chimique,imiyoboro yumuco ikoreshwa ikoreshwa cyane mumico y'utugari, reaction ya chimique, kubungabunga icyitegererezo nibindi bikorwa bikomeye.Akamaro kabo mubikorwa byubushakashatsi ntigishobora kwirengagizwa. Kubera ko utu tubumbe twumuco duhura nubushakashatsi bwikigereranyo, ibikoresho, ingano, gufunga ndetse niyo byahinduwe cyangwa bidafite ingaruka bizagira ingaruka zikomeye kubisubizo byubushakashatsi. Guhitamo nabi bishobora kuganisha ku kwanduzanya, gutakaza icyitegererezo cyangwa kubogama kwamakuru kubogama, bityo bikagira ingaruka kumyororokere no kubyara ubushakashatsi.

Ubwoko Bwingenzi bwumuco wajugunywe

Hariho ubwoko bwinshi bwimiyoboro yumuco ikoreshwa, kandi abashakashatsi bakeneye guhitamo ubwoko bukurikije intego yubushakashatsi, ibidukikije bikora nibiranga ingero. Bashyizwe mu byiciro bitatu bikurikira: ibikoresho, ubushobozi n'imikorere idasanzwe:

1. Gutondekanya kubintu

Imiyoboro yumuco itawe ikozwe mubikoresho bitandukanye iratandukanye mukurwanya ubushyuhe, imiterere yimiti nibintu byiza:

• Polypropylene: Kurwanya ubushyuhe bwinshi hamwe no kwangirika kwimiti, bikwiranye numuco wa selile isanzwe, ubushakashatsi bwibinyabuzima bya molekuline nibindi bikorwa.
• Polystirene: gukorera mu mucyo, byoroshye kwitegereza ibintu byamazi na selile, bikunze gukoreshwa mugupima optique, ariko ntabwo birwanya ubushyuhe bwinshi, mubisanzwe ntibishobora gukoreshwa muri autoclaving.
• Ibirahuri byumuco: Nubwo byakoreshwa kandi bigahinduka imiti, birahenze, bisaba ubundi buryo bwo gukora isuku no kuboneza urubyaro, kandi bikerekana ingaruka zishobora kwanduzwa.

2. Gutondekanya kubushobozi

Ukurikije ingano yicyitegererezo gikenewe mubushakashatsi, ubushobozi bwimiyoboro yumuco buva kuri micro kugeza mubunini:

• Microcentrifuge tubes: bikunze gukoreshwa mugutanga icyitegererezo, imvura igwa, gukuramo ADN / RNA nibindi bikorwa.
• Imiyoboro isanzwe yumuco: ubushobozi bukoreshwa cyane muri laboratoire, bubereye umuco w'akagari, kuvanga reaction, kubika icyitegererezo hamwe nizindi ntego.
• Imiyoboro minini yumuco: bikwiranye n'umuco munini w'akagari cyangwa umubare munini wo gutunganya ibisubizo.

3. Gutondekanya kubikorwa byihariye

Imiyoboro iraboneka hamwe nibintu bitandukanye byinyongera kugirango ihuze ibyifuzo byihariye:

• Aseptic Yabanje kubitsa: uruganda rwahagaritswe nimirasire ya gamma cyangwa autoclaving, ikwiranye nubushakashatsi hamwe nibisabwa byinshi bya aseptic.
• Hamwe na capage: yemerera guhanahana gaze, ibereye mikorobe cyangwa imirongo ya selile isaba guhindagurika kandi ikabuza kwanduza amasoko aturuka hanze.
• Ubushyuhe Buke Kurwanya Imiyoboro: irashobora gukoreshwa neza muri -80 ℃ cyangwa ibidukikije bya azote, bikwiranye no kubika ubushyuhe bwigihe kirekire bwo kubungabunga ingero z’ibinyabuzima.
• Abahawe impamyabumenyi / batarangije: Imiyoboro yahawe impamyabumenyi iroroshye kugereranya no gutanga ingano y'amazi kugirango byongere imikorere yubushakashatsi.

Ibintu byingenzi muguhitamo imiyoboro yumuco

Muburyo bwo gukora igishushanyo mbonera no gukora, gutoranya imiyoboro ikwiye ikoreshwa ni ngombwa kugirango habeho gukora ubushakashatsi no kumenya neza ibisubizo. Abashakashatsi bagomba gusuzuma byimazeyo ibintu byinshi byingenzi:

1. Ubwoko bw'igerageza

Ibisabwa kumiyoboro yumuco iratandukanye cyane mubigeragezo no kugerageza kandi bigomba gutoranywa ukurikije ibikubiye mubikorwa hamwe nibidukikije bigerageza:

• Umuco w'akagari.
• Ubushakashatsi bwa PCR / molekuline.
• Ububiko buke: Imiyoboro ifite ubushyuhe buke bwo hasi igomba gukoreshwa kugirango wirinde lysis kubushyuhe buke.

2. Ibiranga icyitegererezo

Imiterere ya fiziki ya chimique yicyitegererezo igira uruhare rutaziguye mu guhitamo ibikoresho nibikorwa byimiterere yimiyoboro yumuco:

• Ingero zamazi cyangwa zikomeye: igena ingano isabwa nuburyo bwa orifice ya tube.
• Ingero za acide cyangwa alkaline: ingero zangirika cyane zisaba ibikoresho birwanya imiti kugirango wirinde guhindagurika cyangwa kwanduza.
• Kwirinda umucyo: icyitegererezo cyumucyo kigomba guhitamo amber cyangwa opaque material culture tube, kugirango wirinde gufotora.

3. Ibisabwa byo kuboneza urubyaro

Gukenera sterilisation hamwe nubwoko bwa sterisizione ikoreshwa nibyingenzi byingenzi muguhitamo igituba:

• Mbere yo kuboneza urubyaro hamwe no kwifata: uruganda rwabanjirije ibicuruzwa bikwiranye nubushakashatsi bwimbitse, kubika umwanya; niba laboratoire ifite ibikoresho bya autoclave sterilisation, urashobora guhitamo PP tubing ishobora kwikora.
• Guhuza Sterilisation: Kurugero, ibikoresho bya PS ntibikwiriye autoclaving kandi ni kubikoresha rimwe gusa.

4. Guhuza

Imiyoboro igomba guhuzwa neza nibikoresho bya laboratoire kugirango ikore neza nibisubizo byizewe:

• Guhuza Centrifugal: Imiyoboro ikoreshwa muri centrifugation igomba kuba ikomeye muburyo bwo guhangana nimbaraga za centrifugal kumuvuduko mwinshi.
• Guhuza kwikora: Kubushakashatsi busaba gukoresha robot ya pipeti, sisitemu yo gutanga ibyuma byikora, nibindi, hagomba gukoreshwa ingano yubunini busanzwe.

5. Igiciro kandi kirambye

Kugenzura neza ibiciro no gukoresha umutungo nabyo ni ikintu cyingenzi mugukemura ibibazo bikenewe:

• Ikoreshwa rishobora gukoreshwa: imiyoboro ikoreshwa irashobora gucungwa no kwirinda kwanduza, ikwiranye nubushakashatsi bwimbitse; kongera gukoresha ibirahuri bikwiranye nubushakashatsi bwibanze hamwe na bije ntarengwa.
• Igipimo cyo kugura: kugura ingano nini birashobora kugabanya igiciro cyibice, bikwiranye nigihe kirekire, imishinga minini; ntoya-nini, yihariye ibisobanuro biroroshye guhinduka ariko ugereranije nigiciro kinini.

Ibyifuzo Kubisanzwe Bikoreshwa

Ukurikije ibyifuzo byihariye byubwoko butandukanye bwubushakashatsi, imiyoboro yumuco ikurikira irashobora gusabwa kubintu byinshi bisanzwe bikoreshwa, bigamije kunoza imikorere no kwizerwa mubushakashatsi:

1. Guhinga ingirabuzimafatizo

• Ubwoko busabwa: sterile polypropilene yumuco wigituba hamwe na capitif ya karitsiye ihumeka
• Impamvu: Ibikoresho bya polypropilene bifite ubudahangarwa bwimiti nubuzima bwa biocompatibilité, bikwiranye namazi yumuco. Igifuniko cya karitsiye gishobora kumenya guhanahana gaze neza, kwirinda kwanduza mikorobe, no kuzuza ibidukikije bya gaze bikenewe kugirango ikwirakwizwa ry'utugari.

2. PCR / qPCR imenyekanisha ryimikorere

• Ubwoko busabwa: nuclease-yubusa, pyrogen-idafite umuyoboro wihariye wa PCR cyangwa microcentrifuge
• Impamvu: Umuyoboro mwinshi wa polypropilene, nyuma yo kuvurwa cyane, urashobora kwirinda neza kwangirika kwanduye cyangwa kwanduzwa, kugirango habeho ibisubizo nyabyo kandi byizewe. Igishushanyo mbonera kirasabwa kunoza uburyo bwo kohereza ubushyuhe.

3. Kubika ubushyuhe buke

• Ubwoko busabwa: Ubushyuhe Buke Kurwanya Polypropilene Igikoresho hamwe na Anti-Freeze Cracking Igishushanyo hamwe na kashe ya kashe
• Impamvu: Iyi miyoboro irashobora gukoreshwa mukubika ingero umwanya muremure kuri -80 ° C cyangwa no muri azote yuzuye, kandi imiterere yihariye irinda umuyoboro guturika no gutemba. Birakwiye kubika igihe kirekire imirongo ya selile, ingero zamaraso, proteyine cyangwa acide nucleic.

4. Centrifuge

• Ubwoko busabwa: kwihanganira cyane polypropilene tubes, kuzenguruka cyangwa gushushanya hasi, hamwe na rotrifuge
• Impamvu: Imiyoboro ya PP ifite imbaraga zo kurwanya centrifugal kandi irashobora kwihanganira imbaraga zo hejuru zidafite imbaraga zo guhinduka cyangwa guturika. Hasi ya conic ifasha gukusanya selile cyangwa kugwa hagati no kongera igipimo cyo gukira.

Amakosa asanzwe hamwe nuburyo bwo kubyirinda

Mubimenyerezo, kunanirwa mubigeragezo kubera guhitamo nabi imiyoboro yumuco ibaho rimwe na rimwe. Amakosa menshi asanzwe hamwe nibitekerezo bihuye nibisubizo biri kurutonde hepfo kubashakashatsi:

1. Gukoresha nabi ibikoresho bitarwanya ubushyuhe bwo kwihanganira autoclaving