Intangiriro

Mu bushakashatsi bwa siyansi bugezweho no gusesengura igerageza, icyumba cyo gukusanya ingero ni intambwe ya mbere yo kwemeza ko amakuru yizewe. Kandi muri iki gikorwa, uducupa two gukusanya ingero, nk'ikintu cy'ingenzi gitwara ingero mu kubika no gutwara, guhitamo no gukoresha ingero bifitanye isano itaziguye n'ubusugire n'ubudahangarwa bw'ingero ndetse n'ukuri kw'isesengura rikurikiraho.

Uducupa two gukusanya ingero dukoreshwa cyane mu nzego nyinshiUbwoko butandukanye bw'uducupa buratandukanye cyane bitewe n'ibikoresho, imiterere, inyongeramusaruro n'ifungwa ry'ibipimo bitandukanye bifite imiterere itandukanye ya fizikiki, ibikenewe mu isesengura n'imiterere y'ububiko.

Ibyiciro by'ibanze by'amacupa yo gukusanya ingero

Ubwoko butandukanye bw'ibipimo bufite ibisabwa bitandukanye ku macupa yo gukusanya ingero mu gihe cyo kuyakusanya no kuyabika. Kubwibyo, gusobanukirwa ibyiciro by'ibanze by'amacupa yo gukusanya ingero bizafasha abagerageza guhitamo neza hakurikijwe ibyo bakeneye. Muri rusange, imiyoboro y'ingero ishobora gushyirwa mu byiciro hakurikijwe ibikoresho, uburyo bwo kuyifunga n'ingano nk'imiterere y'ibinyabutabire n'imiterere y'imiterere.

1. Gushyira mu byiciro hakurikijwe ibikoresho: ikirahure ugereranyije na pulasitike

• Imiyoboro y'ikirahure: Ubusanzwe ikorwa mu kirahuri cya borosilicate gifite ubushobozi bwo kudakora neza mu binyabutabire no mu bushyuhe, ikwiriye gukoreshwa mu bintu byinshi bisukura ibimera n'ibikoreshwa mu gutunganya ubushyuhe bwinshi. Cyane cyane mu isesengura ryimbitse cyangwa mu gukusanya ibintu byoroshye guhumeka, uducupa tw'ibirahuri dushobora kwirinda kwangirika cyangwa kwanduzwa n'ingero.
• Uducupa twa pulasitiki two gukusanya ingero: Ibikoresho bisanzwe birimo polypropylene, polyethylene, polycarbonate, nibindi. Birwanya ingaruka kandi byoroshye, kandi bikwiranye no gukonjesha, gukusanya ingero z’ibinyabuzima, no gupimwa buri gihe. Amwe mu macupa ya pulasitiki yo mu rwego rwo hejuru nayo arwanya ingese ku rugero runaka.

2. Uburyo bwo gushyira mu byiciro hakoreshejwe uburyo bwo gufunga: screw, bayonet, ubwoko bw'uturemangingo

• Ubwoko bw'imashini: ubwoko bukunze gukoreshwa cyane, bworoshye gufungura no gufunga, bukwiriye ibikenewe muri laboratwari rusange. Udupfundikizo twa screw-port dukunze guhuzwa na gasket za PTFE/silicone kugira ngo harebwe ko dufunga kandi ko duhuye n'imiti.
• Ubwoko bwa bayoneti: Ifungwa vuba hakoreshejwe snap, ikwiriye gukoreshwa vuba cyangwa mu bihe bisaba gufungura kenshi, ikoreshwa cyane cyane mu buryo bwikora cyangwa mu buryo bumwe na bumwe bwo gupima.
• Ubwoko bw'ingirabuzimafatizo: Ifunze n'icyuma gipfundikiye n'agapira, ikoresha umwuka mwinshi cyane, ikoreshwa cyane muri chromatography ya gaze n'ibindi bipimo bisaba kugenzura imihindagurikire y'ikirere. Ikwiriye kubikwa no gutwarwa igihe kirekire, cyane cyane ikoreshwa cyane mu gupima ingero z'ibidukikije.

3. Gushyira mu byiciro hakurikijwe ingano n'imiterere: bisanzwe, bito, hasi hameze nk'inyuguti y'urukiramende, n'ibindi.

• Uducupa dusanzwe: ingano isanzwe ni 1.5 ml, 2 ml na 5 ml, zikwiriye gukusanya no gusesengura ingero nyinshi z'amazi. Imiterere yayo ahanini ni uruziga, byoroshye kuyifata hakoreshejwe ibikoresho byikora.
• Uducupa duto: ifite ingano ya 0.2ml-0.5ml, ikoreshwa cyane ku ngano nto cyane z'ibipimo cyangwa imiterere y'igerageza ifite ubushobozi bwo gukora byinshi. Ikwiriye uburyo bwo gupima ibintu mu buryo buciriritse.
• Uducupa dufite umugozi: Igice cyo hasi cy'agacupa cyakozwe mu ishusho y'agace k'umunyu, byoroshye gukoresha mu gupima ingero, gukoresha centrifugal no gukoresha ibisigazwa by'amazi, ibi bikunze gukoreshwa mu igerageza ryo gukuramo poroteyine/aside nucleic.
• Uducupa two hasi/dukikije two hasi: Uduce duto two hasi tubereye ibikoresho byo gupima by’ikoranabuhanga, mu gihe utwo hasi twizengurutse tubereye cyane mu gukoresha intoki cyangwa mu kuvanga imiyoboro y’amazi.

Ikoreshwa mu Bipimo by'Umubiri (urugero rw'ibipimo by'amaraso)

Nk'imwe mu ngero zisanzwe kandi z'ibanze z'ibinyabuzima, amaraso akoreshwa cyane mu nzego zitandukanye, harimo gusuzuma indwara, gupima imiterere y'uturemangingo, n'ubushakashatsi ku miterere y'amaraso. Bitewe n'imiterere yayo ikomeye n'imikorere ikomeye y'ibinyabuzima, ibisabwa mu bikoresho byo gukusanya ni bikomeye cyane. Uducupa two gukusanya ingero dukoreshwa mu bikorwa bitandukanye turatandukanye mu bijyanye n'inyongera, ibikoresho n'imiterere y'imiterere, ibyo bikaba bigira ingaruka ku buryo butaziguye ku bwiza bw'ingero n'ukuri kw'isesengura rikurikiraho.

1. Ibidukikije n'icyo bigamije

• Gupima ubuvuzi: ku maraso asanzwe, biochimie, electrolytes, isuzuma ry'urugero rw'imisemburo, nibindi, bigomba gukorwa vuba, neza, birinda hemolysis no kwanduzwa.
• Ubushakashatsi ku binyabuzima bya molekile: nka RNA-seq, sequencing ya genome yose (WGS), qPCR, nibindi, bisaba ibisabwa cyane kugira ngo aside nucleic ikomeze kuba nziza no kubungabunga ingero.
• Isesengura rya poroteyine n'imikorere y'umubiri: bahangayikishijwe no gukumira ibikorwa bya poroteyine, guhuza imiterere y'ibinyabutabire, kudahungabana nyuma yo gukonjesha no gushonga kenshi.

2. Ubwoko bw'uducupa dusanzwe two gukusanya ingero n'imiterere yatwo

• Irimo uducupa duto two kurwanya amaraso: Imiyoboro ya EDTA ikoreshwa cyane mu gupima amaraso no gukuramo aside nucleic, bishobora kubuza neza inzira yo gufungana no kurinda imiterere y'uturemangingo; Imiyoboro ya Heparin ikwiriye gusesengura plasma, ikwiriye gupimwa bimwe na bimwe bya shimi (urugero, gusesengura imyuka mu maraso), ariko ikabangamira ibikorwa bimwe na bimwe bya PCR; kandi imiyoboro ya Sodium citrate ikoreshwa cyane mu gupima imikorere yo gufungana.
• Imiyoboro yo gukusanya amaraso y'umwimerere idafite inyongeramusaruro: ikoreshwa mu gupima amaraso mu maraso, nko gufata akazi, imikorere y'impyiko, ibizamini by'ubudahangarwa bw'umubiri, nibindi. Nyuma y'uko amaraso apfutse mu buryo busanzwe, amaraso atandukanywa na centrifugation kugira ngo hirindwe ko imiti yongeramo ibinyabutabire yabangamira uburyo bwo gukora igerageza.
• Uducupa twihariye two kubika amazi mu buryo bwa cryopreservation: ikozwe mu bikoresho bya PP bikomeye cyane, ishobora kwihanganira ubushyuhe buri hasi cyane (-80℃ kugeza ku bidukikije bya azote y'amazi). Ikunze gukoreshwa mu kubungabunga plasma, serum, ibice bya selile igihe kirekire, nibindi, ikoreshwa cyane mu bubiko bw'ibipimo by'ibinyabuzima no mu bushakashatsi bw'igihe kirekire.

3. Icyitonderwa

• Ingaruka z'ibikoresho ku guhagarara kw'icyitegererezo: Uducupa twa pulasitiki dushobora kwinjiza poroteyine cyangwa aside nucleique, bigasaba gukoresha ibikoresho bike byo kwinjiza cyangwa gutunganya ubuso. Uducupa tw'ikirahure turahamye ariko ntidukwiriye mu bihe byose byo gukonjesha Abatekinisiye ba laboratwari bagomba gufata icyemezo bashingiye ku miterere y'icyitegererezo n'ibyo igerageza rikeneye.
• Akamaro ko gushyira ibirango no gukurikirana: Mu gihe cy'igerageza, ingero ziroroha kwitiranya bitewe n'ibirango, amakuru atuzuye n'ibindi bibazo, bigira ingaruka zikomeye ku kwizerwa kw'amakuru. Ni byiza gukoresha inyuguti zanditseho laser, stika ziramba zo kubika cyangwa sisitemu yo gukurikirana ibintu hakoreshejwe ikoranabuhanga (nka RFID, barcode) mu gikorwa cyose cyo gucunga ingero.

Kubika no gufata ingero z'amaraso bigira uruhare runini mu bisubizo by'igerageza, kandi uducupa dukwiriye two gukusanya ingero ntabwo twongera gusa ubuziranenge bw'ibikorwa by'ingero, ahubwo ni n'ingenzi mu bushakashatsi bwo ku rwego rwo hejuru. Bitewe n'iterambere ry'ubuvuzi bunoze n'ikoranabuhanga rigezweho, gukenera uducupa two gukusanya ingero z'ibinyabuzima bigenda birushaho kuba amazi kandi binonosorwa.

Isesengura ry'imiti n'ingero za laboratwari

Mu buhanga bwo gusesengura shimi, kuvumbura imiti, gupima umutekano w'ibiribwa n'indi mirimo yo muri laboratwari, uducupa two gukusanya ingero ntabwo ari ibikoresho byo kubikamo gusa, ahubwo ni n'ibice bihuza cyane mu buryo bwo gusesengura hamwe n'igikoresho. Cyane cyane muri chromatography y'amazi, chromatography ya gaze n'ubundi buryo bwo gupima neza, guhitamo uducupa bifitanye isano itaziguye no kongera gusesengura no gukora neza kw'isesengura ndetse n'imikorere ihamye y'igikoresho.

1. Gukoresha utubari mu gusesengura chromatography y'amazi no gusesengura chromatography ya gaze

• Uducupa twa HPLC: hakenewe uburyo bwiza bwo guhagarara neza mu binyabutabire kugira ngo umuti w’icyitegererezo udakora cyangwa ngo winjire ku rukuta rw’agacupa. Ubusanzwe hakoreshwa uducupa tw’ikirahure twa mililitiro 2 dufite imipfundikizo ya PTFE/silicone gasket, turwanya imiti y’umwimerere kandi tugafata neza. Ku bipimo byoroshye kubona amafoto, hari amacupa y’umukara.
• Uducupa twa GC: kubera ko isesengura rya GC rishingiye cyane ku guhinduka kw'icyitegererezo, utu ducupa tugomba gufungwa neza kandi akenshi tuba dukozwe mu ducupa tw'ikirahure dufite imipfundikizo y'umuvuduko; byongeye kandi, kugira ngo hirindwe ko ibice bihinduka, utu dupfundikizo twabanje gukubitwa akenshi dukoreshwa mu gufunga udupfundikizo twa aluminiyumu.
• Porogaramu zifite ingero nto: Kugira ngo hamenyekane uburyo bwo gupima no gutahura ibice by’ibice, utuntu duto twa mililitiro 0.3-0.5 dushobora gukoreshwa hamwe na cannulae kugira ngo tugabanye igihombo cy’icyitegererezo.

2. Ingano ya sisitemu yo gukora isuzumabumenyi ryikora n'ibisabwa kugira ngo ihuzwe n'uburyo ikoreshwa

Laboratwari zigezweho zikunze gukoresha ibikoresho byo gupima kugira ngo byongere imikorere myiza no guhuza neza, kandi hari amahame amwe atangwa ku bipimo by'ibicupa n'imiterere yabyo:

• Ibipimo bisanzwe: 2ml isanzwe ya caliber (OD 12mm * Uburebure 32mm) niyo moderi nyamukuru yo kuzenguruka kw'ibinyabiziga, ijyanye cyane na sisitemu yo gupima ibikoresho by'ubwoko bwinshi.
• Ibisabwa kugaragara kw'ikiyiko: umunwa w'icupa ugomba kuba ugororotse, umubiri w'icupa ugomba kuba urwanya uburyaryate bwa mashini, kugira ngo icyuma gifata ukuboko gihamye.
• Guhindura ububiko bwihariye: Amwe mu moko y'ibicuruzwa akenera imiterere yihariye yo hasi (hepfo irambuye, hepfo izengurutse, cyangwa ifite imiyoboro) kugira ngo ihuze n'agacupa k'ibirayi.

3. Ibikoresho byihariye n'igishushanyo mbonera cy'imikorere

Kugira ngo hamenyekane neza ko ingero zigoye zikora neza, laboratwari zikunze gukoresha uducupa twabugenewe:

• Ikirahure cya borosilicate kidakora neza: Ni agacupa gakundwa cyane kuri HPLC/GC kubera ko gafite ubushobozi bwo kurwanya ibinyabutabire byinshi n'isuku, karinda ingaruka cyangwa kwinjizwa mu bipimo by'ibice by'ifatizo.
• Umupfundikizo wa gasket wa PTFE: kurwanya ingese nyinshi, gutobora bishobora kongera gukoreshwa, bikwiye gukoreshwa mu gupima urushinge mu buryo bwikora, birinda kwanduza ingero no kuva amazi.
• Icupa ryo kuvura silanization: ubuso buterwaho irangi ridasanzwe kugira ngo bigabanye ukwinjira kwa molekile zo mu gice cy'isi, rikoreshwa cyane mu gusesengura ibimenyetso.

Mu guhitamo ibikoresho bikwiye, imiterere n'ibipimo, uducupa two gusesengura imiti ntitwongera gusa ubushobozi bwo gupima no guhuza ibikoresho, ahubwo tunagira uruhare mu gutuma amakuru y'icyitegererezo ahora ahindagurika kandi yizewe. Cyane cyane mu isesengura ry'ibice n'imikorere yikora, imiterere ikwiye ya sisitemu z'uducupa yabaye imwe mu ngingo z'ingenzi zigaragaza ubuziranenge bw'ikizamini.

Guhitamo uducupa mu ikusanyirizo ry'ibikomoka ku bidukikije

Gukusanya ingero z’ibidukikije bikubiyemo ibintu byinshi bitandukanye, nk'amazi, ubutaka n'ikirere, kandi ingero zifite imiterere igoye kandi zishobora kuba mu bihe bikomeye (urugero: kwangirika cyane, guhindagurika cyane, ibintu bihumanya ikirere, nibindi). Kugira ngo amakuru yakusanyijwe abe yizewe kandi hubahirizwe ibisabwa n'amategeko, ni ngombwa guhitamo uducupa dukwiye two gukusanya ingero.

1. Imiterere y'ikoreshwa

• Ingero z'amazi: Amazi yo hejuru ya Baokou, amazi yo munsi y'ubutaka, amazi yanduye yo mu nganda, n'ibindi, bikunze gukoreshwa mu gutahura ibyuma biremereye, imyanda ihumanya ikirere. Umunyu w'intungamubiri, n'ibindi.
• Ibikomoka ku butaka: ingero z'amazi zabonetse hakoreshejwe imiti ikoreshwa mu gukuramo imiti, irimo ibisigazwa by'imiti yica udukoko, ibyuma biremereye cyangwa ibintu bikomoka ku bimera.
• Amazi yo gukusanya uduce duto tw’umwuka: ingero z'udukoko dukoreshwa mu gusohora mu tuyunguruzo cyangwa mu binyobwa bikurura uduce tw'amazi.

2. Ibisabwa by'ingenzi ku macupa yo gufatiraho ingero

• Gufunga neza: Irinde ko ingero zihinduka, amazi ava cyangwa amazi ashira mu gihe cyo kuzitwara cyangwa kuzibika, cyane cyane ingenzi mu gupima VOC.
• Ubudahangarwa bwiza bwo kwangirika: ingero zishobora kuba zirimo aside ikomeye, alkali cyangwa solvents organic, bisaba gukoresha ikirahure cya borosilicate cyangwa amacupa akozwe muri pulasitiki yihariye.
• Igishushanyo mbonera kidasobanutse: Urukuta rw'imbere rw'agacupa rugomba kwirinda guhumeka ibintu bihumanya cyangwa ngo bihure n'ibice by'icyitegererezo, kandi isesengura rimwe na rimwe risaba gukoresha amacupa yasizwe silani cyangwa yakarabye mbere.
• Iyubahirizwa ry'amabwiriza agenga ingero: Ubwoko bwose bwa gahunda zo kugenzura ibidukikije bukunze kugengwa n'amahame y'igihugu cyangwa mpuzamahanga, nka EPA ya Amerika, Amahame y'Ubuziranenge bw'Ibidukikije mu Bushinwa ku mazi yo hejuru, n'ibindi, kandi uducupa tugomba gutoranywa kugira ngo twuzuze ibisabwa mu gupima.

Uducupa twatoranijwe nabi dushobora guteza ibibazo nko kwangirika kw'ibintu byibandwaho, kwinjiza imyanda, n'ahantu hadafite imiterere, bishobora kugira ingaruka ku buryo ibisubizo by'ibizamini bitoroshye cyangwa bigatera ikosa ry'amakuru. Kubwibyo, guhitamo utwuma tw'icyitegererezo mu buryo bwa siyansi kandi bukwiye mu igenzura ry'ibidukikije ntabwo bifitanye isano gusa n'ubwiza bw'isesengura, ahubwo binafitanye isano no kubahiriza amategeko no gufata ibyemezo bya siyansi mu bijyanye n'ibidukikije.

Inyigisho yo Guhitamo Icupa: Uburyo bwo Guhitamo Hashingiwe ku Rugero rw'Icyitegererezo n'Ibikenewe mu Isesengura

Nubwo hari ubwoko bwinshi bw'uducupa two gukusanya ingero, uburyo bwo guhitamo neza kandi mu buryo bwa siyansi mu kazi gasanzwe ni ikibazo gikunze kugaragara ku bashakashatsi mu bushakashatsi.

1. Imiterere y'icyitegererezo niyo igena uburyo ibikoresho by'ibanze bifatwa

• Imiterere y'icyitegererezo ubwacyo: Niba icyitegererezo gihindagurika cyane, icupa ry'ikirahure rifite umupfundikizo mwiza wo gufunga hamwe na gasket ya PTFE ni byo byiza kugira ngo ibice byaryo birusheho gutakaza. Mu gihe hari icyitegererezo gikomeye cyangiza, ugomba gukoresha amacupa y'ikirahure ya borosilicate adakoresha imiti, cyangwa ugakoresha polyethylene ifite ubucucike bwinshi, plastiki ifite fluorine n'ibindi bikoresho byihariye bizwi nka icupa. Byongeye kandi, ku bipimo bikora neza mu mubiri bikungahaye kuri aside nucleic, poroteyine cyangwa mikorobe, hagomba gukoreshwa uducupa tudafite enzymes kandi twavuwe na aseptic, kandi ibikoresho bidakoresha enzymes cyangwa bidafite aside ni byiza cyane kugira ngo hirindwe kwangirika cyangwa kongerwamo amazi mu buryo budasanzwe.
• Ubwoko n'uburyo ibikoresho by'isesengura bihura: Sisitemu yo gupima ikoreshwa mu buryo bwikora, igomba kwemeza ko ingano y'agacupa gakoreshwa, uburyo umunwa w'icupa umeze neza, ubunini bwa gasket, nibindi bihuye n'ibipimo bisanzwe by'uruganda rukora ibikoresho. Ubusanzwe, icupa ry'ikirahure rya mililitiro 2 rikoreshwa kugira ngo harebwe ko urushinge rwo guterwamo rugenda rukurwamo buhoro buhoro no kwirinda kuziba cyangwa gusohoka kw'urushinge. Ku bijyanye no gupima cyangwa gutanga ibizamini n'intoki, ubwoko bw'agacupa bworoshye ni bwo bwiza.
• Uburyo bwo kubika ingero: Uburyo ibikwamo by’icyitegererezo bigira ingaruka ku buryo butaziguye ku ihitamo ry’ibikoresho n’imiterere y’icyitegererezo. Uducupa twinshi tw’ikirahure cyangwa polypropylene turahagije ku bipimo bisanzwe bifungishwa muri firigo igihe gito. Niba ibipimo bigomba kubikwa ku bushyuhe buri hasi (-20℃ cyangwa -80℃), hagomba gukoreshwa imiyoboro yihariye yo gukonjesha, ikozwe muri PP idashyuha cyane kandi ifite imiterere yo gufunga O-ring irinda kuva. Niba ibipimo bibitswe muri azote y’amazi igihe kirekire, hagomba gukoreshwa uducupa twihariye twa azote y’amazi, kandi hagomba gukoreshwa udupfundikizo cyangwa udupfundikizo tw’imbere two gukonjesha kugira ngo dukingwe kugira ngo tutubike. Byongeye kandi, ku bikoresho bishobora kwangirika, uducupa tw’umukara cyangwa tudafite ishusho tugomba gukoreshwa cyangwa gushyirwaho ibikoresho byo kubika bidatanga urumuri.
• Ingano y'ikiguzi n'ingano y'igerageza: Ku igerageza rigezweho cyangwa laboratwari zo kwigisha, uducupa twa pulasitiki duhendutse dushobora guhitamo kugira ngo tugabanye ikiguzi cyo gukoresha. Ku isesengura ry’ukuri cyangwa gufata ingero z’agaciro kanini, hagomba kwibandwa ku isuku y’amacupa, kudakoresha ibikoresho, no gufunga neza, bishobora gufasha kwemeza ko amakuru ari ukuri nubwo byaba bihenze gato. Byongeye kandi, mu gihe wubaka amasomero y’icyitegererezo cyangwa mu gucunga imishinga y’igihe kirekire, ni ngombwa gushyira imbere uducupa tw’icyitegererezo dufite barcode, twirinda firigo, kandi turwanya umwanda kugira ngo twongere imikorere myiza yo gukurikirana ingero no gucunga amakuru.

Iyo ushyize hamwe, agacupa k'icyitegererezo, nubwo ari gato, ni isano ikomeye hagati y'igishushanyo mbonera cy'igerageza, ubwiza bw'icyitegererezo n'ibisubizo by'isesengura. Binyuze mu gusuzuma imiterere y'icyitegererezo, ibikoresho by'igerageza, uburyo bwo kubika hamwe n'ingano y'ingengo y'imari, isoko y'ubushyuhe bw'igerageza ishobora guhitamo neza agacupa k'icyitegererezo gakwiriye, bigashyiraho urufatiro rukomeye rw'igikorwa cyose cy'ubushakashatsi.

Ibyerekezo by'ejo hazaza n'icyerekezo gishya

Bitewe n’iterambere ry’ubushakashatsi bwa siyansi bwikora ndetse n’igitekerezo cy’igerageza ry’icyatsi kibisi, uducupa two gukusanya ingero turimo gutera imbere mu rwego rwo kugumana uburyo busekeje kandi bwo kurengera ibidukikije.

Ku ruhande rumwe, laboratwari zikora cyane zifite ibisabwa byiyongera kugira ngo habeho umuvuduko n'ubucucike bw'ibipimo, bigatuma uducupa dutangira kugenda buhoro buhoro tugana ku buryo bwo gukora buhoro no gukoresha uburyo bwo gukora. Uducupa duto duto turimo kuba ingenzi, kandi iyo dukoreshejwe na sisitemu zikora, ntitwizigama umwanya n'ibikoresho gusa, ahubwo tunanoza imikorere myiza, duhuza n'ibyo igerageza rimaze kugeraho risaba kugira umuvuduko n'ubuhanga.

Ku rundi ruhande, ubushakashatsi n'iterambere ry'ibikoresho birengera ibidukikije nabyo byabaye intego y'inganda. Kugira ngo bagabanye umutwaro w'ibidukikije uterwa na pulasitike zikoreshwa mu gihe cyo kuzikoresha, uducupa twinshi turimo gukoresha ibikoresho bishobora kwangirika cyangwa bishingiye ku binyabuzima. Muri icyo gihe, uburyo bwo gupakira no gukora bukunda koroshya kandi bugatera icyatsi kibisi, hakurikijwe icyerekezo cy'ubwubatsi burambye bwa laboratwari.

Mu gihe kizaza, amacupa ntabwo azaba ari ayo gukoresha gusa, ahubwo azaba n'igice cy'ingenzi mu iterambere ry'ubwenge kandi rirambye rya laboratwari.